Part:BBa_K2623004

SasA coding region

Summary

It is involved in signal transduction. Participates in the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria, via its interaction with KaiC. Required for robustness of the circadian rhythm of gene expression and is involved in clock outputs.
As part of the Kai circuit, phosphorylated KaiC can phosphorylate SasA, allowing SasA to participate in the downstream signaling pathway as a histidine kinase. Phosphorylated SasA can phosphorylate RpaA which acts as a specific transcription factor to participate in the expression of related genes.
A separate fragment is 1164 bp.

Structure of SasA

Fig.1 Protein structure of SasA

SasA's function

Fig.2 Simplified model of the central circadian oscillator and output activities.[1]

Reference

[1]Joseph S. Markson,Joseph R. Piechura,Anna M. Puszynska,Erin K. O’Shea. Circadian Control of Global Gene Expression by the Cyanobacterial Master Regulator RpaA[J]. Cell,2013,155(6).
More information about our project can be found on our results page.http://2018.igem.org/Team:XMU-China/Results
Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 538
  Illegal BglII site found at 607
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]